Development of a Biosensor for Environmental Monitoring Based on Microalgae Immobilized in Silica Hydrogels
نویسندگان
چکیده
A new biosensor was designed for the assessment of aquatic environment quality. Three microalgae were used as toxicity bioindicators: Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii. These microalgae were immobilized in alginate and silica hydrogels in a two step procedure. After studying the growth rate of entrapped cells, chlorophyll fluorescence was measured after exposure to (3-(3,4-dichlorophenyl)-1,1-dimethylurea) (DCMU) and various concentrations of the common herbicide atrazine. Microalgae are very sensitive to herbicides and detection of fluorescence enhancement with very good efficiency was realized. The best detection limit was 0.1 µM, obtained with the strain C. reinhardtii after 40 minutes of exposure.
منابع مشابه
Alginate/silica hybrid materials for immobilizationof green microalgae Chlorella vulgaris for cellbasedsensor arrays
"Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cellbased sensor arrays" (2014). Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hy...
متن کاملDesign and Fabrication of Biosensor Based on Immobilized AchE on Modified Electrode by Graphene-multiwall Carbon Nanotubs/Beta Cyclodexterin-chitosan
Organophosphorus (OP) forms an important class of toxic compounds. They inhibit acetyl cholinesterase (AChE, EC 3.1.1.7) that results in respiratory and myocardial malfunctions. Pesticides could be accumulated in vegetables and fruits, so detection of them is very important. The goals of this study are decreasing detection time and detection limit of methyl parathion bioprobe. In this research ...
متن کاملA Large Response Range Reflectometric Urea Biosensor Made from Silica-Gel Nanoparticles
A new silica-gel nanospheres (SiO2NPs) composition was formulated, followed by biochemical surface functionalization to examine its potential in urea biosensor development. The SiO2NPs were basically synthesized based on sol-gel chemistry using a modified Stober method. The SiO2NPs surfaces were modified with amine (-NH2) functional groups for urease immobilization in the presence of glutaric a...
متن کاملModified Glass Carbon Electrode (GCE) Electropolymerized Polypyrrole Nanofibers with Hemoglobin (Hb) Film as a Unique Biosensor for Nitrite Determination
Abstract: In this study, we were investigated behavior the electrochemical reductionof nitrite at a hemoglobin (Hb) immobilized on glass carbon electrode (GCE) containingpolypyrol nanofiber (ppy) films. Polypyrrole (PPy) nanofibers have been constructed onGCE applying electrochemical technique, and can to deposit diverse polymers onminiaturized electrodes with this commo...
متن کاملFast Monitoring of the Phosphate Ions at Sub-mg L level with the Aim of Diamine-grafted MCM-41 Mesoporous silica and Ion Chromatography
In this work, an innovative method is described for the preconcentration of phosphate ions using ethylenediamine functionalized mesopor (MCM-41). Functionalized MCM-41 was synthesized and the presence of organic groups in the silica framework was demonstrated by FTIR spectrum. The amount of organic groups immobilized on silica surface was determined by elemental analysis and TGA. The functi...
متن کامل